Environment in kill room consistently tested positive for Salmonella and E.coli with APC counts in the millions (1.5 -2.75 x 106). PURE Hard Surface was applied to a cleaned wall adjacent to line, where highest counts were recorded.

Results:

After application of PURE Hard Surface, there were consistently low bacterial counts with reductions ranging from 3-6 log. Testing at a 10-day mark remained negative for Salmonella and only 9 CFU for E. coli.

The processing environment in a RTE facility producing and packaging sliced meat with BBQ sauce in a vacuum sealed pack was finding consistent positive tests for Listeria and E. coli, but had been unable to pinpoint the source. PURE was called in to help evaluate the production and sanitation process and make recommendations for application of PURE Hard Surface to resolve the issue. Our technical team was able to identify the elusive source of contamination through a systematic process and developed a treatment plan for the facility. PURE Hard Surface was applied at pre-operation and shift change to multiple food contact surfaces of food processing equipment, food transfer trolleys, trolley wheels and food packaging equipment.

Results:

A cleaning/disinfecting schedule was implemented with application of PURE Hard Surface twice daily. Treatment of the slicer, transfer trolley and blending table were the focus, however, multiple surfaces throughout the processing facility were treated. Retesting of treated surfaces after four (4) days following this schedule showed that both Listeria and E. coli counts in the facility were negative. The plant was able to maintain these results during future operation following this schedule.

Plant was shut down by FDA for Listeria contamination throughout facility. PURE Bioscience was called in for remediation. Facility was cleaned and sanitized (disinfected) using PURE Bioscience SOPs with PURE Hard Surface.

Results:

The FDA pulled 300 environmental swabs from throughout facility following remediation with PURE Hard Surface and could not find a single positive test for Listeria contamination. (published – James Marsden)

Spotlight -Listeria Remediation

KSU Listeria Remediation Report

A cannabis company growing flower products and producing edibles was experiencing reoccurring challenges with microorganisms in their air handling, drains, and curing/drying room environments. With increased pressure to keep their products as safe as possible, the company was looking for help to reduce Listeria spp. in the facility, clean the drains and lower total APC counts in the curing/drying rooms. They wanted a solution that lowered their water usage and use of toxic chemistries. The company learned about PURE’s success in produce processing and reached out for help.

PURE's team helped develop a treatment plan to apply PURE Hard Surface to environmental and product contact surfaces and PURE Hi-Foam cleaner in the drains. Treatments were applied in between the curing of batches and growth cycles of product.

Results:

The treatment plan was able to control the total APC in the environment and on product contact surfaces, eliminate Listeria spp., and keep the drains clean. The results were so outstanding that the plant expanded use to all 15 + curing rooms in each of its three facilities mirroring strong protocols followed by large produce companies using PURE’s innovative solutions.

A multi-site evaluation was conducted at a Quick Serve Restaurant chain. PURE Hard Surface replaced the current sanitizer without change of corporate in-store SOPs, in the kitchen, food preparation and dining areas. During the three-month evaluation, control stores were tested concurrently using the current chain sanitizer. Corporate protocol stated that APC counts higher than a specific level were considered to be a reportable “incident”.

Results:

APC samples taken from control locations where the sanitizer remained unchanged observed reportable incidents in 25% on average. Locations using PURE Hard Surface observed reportable incidents in only 0.9% on average of APC samples taken.

PURE Hard Surface replaced the current sanitizer without changing corporate in-store SOPs in multiple restaurant locations. Use at the bar, food preparation areas and dining areas of this restaurant chain was evaluated for two-months. Control stores were tested concurrently using the standard sanitizer.

Results:

APC swab results of the locations using the standard sanitizer showed an average count of 8,985 CFU with only 10% of all swabs testing negative for growth. The APC swab results of the locations using PURE Hard Surface showed an average count of 30 CFU with 93% of all swaps being negative – NO GROWTH.

PURE worked directly with a quick-service restaurant to evaluate and improve sanitation throughout the establishment. The tomato slicer was identified as a particularly challenging piece of equipment to clean and sanitize. The slicer is meant to save time in produce preparation, however, disassembly and reassembly for cleaning and sanitizing was time consuming, not to mention the sharp blades made thorough sanitation a dangerous task. As a result, inadequate sanitation was a concern for this customer.

PURE compared cleanliness of the slicer, using criteria defined by the customer, following standard cleaning and sanitization to a new process that implemented application of PURE Hard Surface to the slicer after cleaning.

Results

PURE Hard Surface applied to the tomato slicer outperformed the standard cleaning and sanitizing practices and exceeded the acceptable cleanliness criteria set by the customer.

Tomato Slicer Summary Report

Evaluations were conducted comparing standard cleaning and sanitization of various surfaces within three Assisted Care Living Facilities followed by application of PURE Hard Surface. Following standard cleaning and sanitizing SOPs, APC swabs were taken and enumerated as control samples. PURE Hard Surface was then applied according to PURE Bioscience SOP (mist surfaces, do not wipe) and APC swabs were taken again for evaluation.

Results:

Control swabs showed a combined total count after standard cleaning and sanitizing of 383,700 CFU for the three test facilities. PURE Hard Surface Evaluation swabs showed a combined total count of 1,380 CFU for the three test facilities - >99% reduction.

A California State Prison Hospital in Stockton, California, experienced continued outbreaks of MRSA and Norovirus. PURE Bioscience SOPs were instituted for cleaning and disinfecting the 2,951 bed facility.

Results:

After implementation of PURE Hard Surface, following the suggested SOPs, the prison hospital has had NO MRSA or Norovirus outbreaks for 6 years counting.

Multiple Food Distribution companies are using PURE Hard Surface following suggested SOPs to meet FSMA required cleaning and sanitizing guidelines. Evaluation was conducted at a Food Distributor location in Florida during the summer months. A cleaned and sanitized trailer, using old SOPs was brought to the PURE team. APC swabs were pulled as control samples prior to treating with PURE Hard Surface. The 53 foot trailer was misted for 4 minutes and APC swabs were taken immediately following application, 5 days later and 15 days later without any further remediation. This truck was in use daily delivering produce.

Results:

Initial APC counts prior to PURE Hard Surface application (on a cleaned and sanitized trailer) were 842,630 CFU. APC counts following the initial 4 minute application of PURE Hard Surface were 700 CFU. After returning to use, testing at five days following application, APC was 7,600 CFU and fifteen days following application, APC was 41,600 CFU.